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Neottiglossa trilineata is elongate, oval and 5.5-6.0 mm long. The head is black or brownish black with deep black punctures. It is triangularly shaped lacking swollen juga. The pronotum and scutellum have a pale median line that extends from base to apex. The elytra and base of pronotum are dull yellow. The legs are yellow with femora and tibia having black lines or spots. The beak just about reaches hind coxae. Neottiglossa trilineata can be distinguished from N. undata because of its larger size darker colouring and absence of line on clypeus (McPherson 1982).

Neottiglossa trilineata ranges from Nova Scotia west to British Columbia (McPherson 1982). It ranges across the United States with its northern most range in Alaska and southern most range in California (Scudder 2004).